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The influence of 8-prenylnaringenin on the activity of voltage-gated kv1.3 potassium channels in human jurkat t cells

Cellular & Molecular Biology Letters volume 17, pages 559–570 (2012)Cite this article

Abstract

Using the whole-cell patch-clamp technique, we investigated the influence of 8-prenylnaringenin on the activity of the voltage-gated Kv1.3 potassium channels in the human leukemic T lymphocyte cell line Jurkat. 8-prenylnaringenin is a potent plant-derived phytoestrogen that has been found to inhibit cancer cell proliferation. The results show that it inhibited the Kv1.3 channels in a concentration-dependent manner. Complete inhibition occurred at concentrations higher than 10 μM. The inhibitory effect of 8-prenylnaringenin was reversible. It was accompanied by a significant acceleration of channel inactivation without any pronounced change in the activation rate. Of the naringenin derivatives tested to date, 8-prenylnaringenin is the most potent inhibitor of the Kv1.3 channels. The potency of the inhibition may be due to the presence of a prenyl group in the molecule of this flavonoid. The inhibition of the Kv1.3 channels might be involved in the antiproliferative and pro-apoptotic effects of 8-prenylnaringenin that have been observed in cancer cell lines expressing these channels.

Abbreviations

Iprel :

relative peak current

Kv:

voltage-gated potassium channels

Tmax :

time needed for the recorded currents to reach their maximal value

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Authors and Affiliations

  1. Department of Biophysics, Wrocław Medical University, ul. Chałubińskiego 10, 50-368, Wrocław, Poland

    Justyna Gąsiorowska, Andrzej Teisseyre, Anna Uryga & Krystyna Michalak

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  1. Justyna Gąsiorowska
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  2. Andrzej Teisseyre
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  3. Anna Uryga
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  4. Krystyna Michalak
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Correspondence to Andrzej Teisseyre.

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Gąsiorowska, J., Teisseyre, A., Uryga, A. et al. The influence of 8-prenylnaringenin on the activity of voltage-gated kv1.3 potassium channels in human jurkat t cells. Cell Mol Biol Lett 17, 559–570 (2012). https://doi.org/10.2478/s11658-012-0029-0

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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