- Research article
Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation
Cellular & Molecular Biology Letters volume 18, pages 163–186 (2013)
Mesenchymal stem cells (MSCs) are multipotent cells that are able to differentiate into mesodermal lineages (osteogenic, adipogenic, chondrogenic), but also towards non-mesodermal derivatives (e.g. neural cells). Recent in vitro studies revealed that, in the absence of any kind of differentiation stimuli, undifferentiated MSCs express neural differentiation markers, but the literature data do not all concur. Considering their promising therapeutic potential for neurodegenerative diseases, it is very important to expand our knowledge about this particular biological property of MSCs. In this study, we confirmed the spontaneous expression of neural markers (neuronal, glial and progenitor markers) by undifferentiated human MSCs (hMSCs) and in particular, we demonstrated that the neuronal markers βIII-tubulin and NeuN are expressed by a very high percentage of hMSCs, regardless of the number of culture passages and the culture conditions. Moreover, the neuronal markers βIII-tubulin and NeuN are still expressed by hMSCs after in vitro osteogenic and adipogenic differentiation. On the other hand, chondrogenically differentiated hMSCs are negative for these markers. Our findings suggest that the expression of neuronal markers could be common to a wide range of cellular types and not exclusive for neuronal lineages. Therefore, the expression of neuronal markers alone is not sufficient to demonstrate the differentiation of MSCs towards the neuronal phenotype. Functional properties analysis is also required.
adipogenic induction medium
adipogenic maintenance medium
bovine serum albumin
cyclic adenosine monophosphate
Dulbecco’s modified Eagle’s medium
fluorescence-activated cell sorting
fetal bovine serum
dorsal root ganglia
forward light scatter
human mesenchymal stem cells
nerve growth factor
neural progenitor basal medium
neuron restrictive silencer factor
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Foudah, D., Redondo, J., Caldara, C. et al. Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation. Cell Mol Biol Lett 18, 163–186 (2013). https://doi.org/10.2478/s11658-013-0083-2
- Mesenchymal stem cells
- Neural markers
- Osteogenic differentiation
- Adipogenic differentiation
- Chondrogenic differentiation
- Neuronal differentiation