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Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions


Excessive trafficking of leukocytes can lead to serious tissue injury. Here, four regioselectively sulfated chitosans were assessed as inhibitors of HL-60 leukocyte binding to P-selectin, by investigating their effect on leukocyte adhesion to CHO cells expressing human P-selectin under static and flow conditions. The results show that the sulfochitosans exhibit inhibitory activity in this general order: heparin > N-sulfated/6-O-sulfated chitosan ≥ 3-O,6-O-sulfated chitosan > 6-O-sulfated chitosan N-sulfated chitosan. This suggests that the sulfation of the double site in chitosan is essential for efficient inhibition of P-selectin-mediated HL-60 leukocyte adhesion and that such sulfochitosans may have potential as therapeutic agents against inflammatory disease.



a non-blocking mAb against P-selectin




fetal bovine serum


high performance liquid chromatography


N-sulfated chitosan


N-sulfated/6-O-sulfated chitosan


recombinant human P-selectin/Fc chimera protei


3-O,6-Osulfated chitosan


6-O-sulfated chitosan


a leukocyte adhesion blocking IgG mAb against P-selectin


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Correspondence to Xianlu Zeng or Min Wei.

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Huang, J., Wang, R., Liu, X. et al. Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions. Cell Mol Biol Lett 18, 200–208 (2013).

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Key words

  • Chitosan
  • Sulfated chitosan
  • P-selectin
  • Inhibition
  • Cell adhesion
  • Flow condition
  • Heparin