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Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions
Cellular & Molecular Biology Letters volume 18, pages 200–208 (2013)
Excessive trafficking of leukocytes can lead to serious tissue injury. Here, four regioselectively sulfated chitosans were assessed as inhibitors of HL-60 leukocyte binding to P-selectin, by investigating their effect on leukocyte adhesion to CHO cells expressing human P-selectin under static and flow conditions. The results show that the sulfochitosans exhibit inhibitory activity in this general order: heparin > N-sulfated/6-O-sulfated chitosan ≥ 3-O,6-O-sulfated chitosan > 6-O-sulfated chitosan ≫ N-sulfated chitosan. This suggests that the sulfation of the double site in chitosan is essential for efficient inhibition of P-selectin-mediated HL-60 leukocyte adhesion and that such sulfochitosans may have potential as therapeutic agents against inflammatory disease.
a non-blocking mAb against P-selectin
fetal bovine serum
high performance liquid chromatography
recombinant human P-selectin/Fc chimera protei
a leukocyte adhesion blocking IgG mAb against P-selectin
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Huang, J., Wang, R., Liu, X. et al. Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions. Cell Mol Biol Lett 18, 200–208 (2013). https://doi.org/10.2478/s11658-013-0084-1
- Sulfated chitosan
- Cell adhesion
- Flow condition