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Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions

Cellular & Molecular Biology Letters volume 18, pages 200–208 (2013)Cite this article

Abstract

Excessive trafficking of leukocytes can lead to serious tissue injury. Here, four regioselectively sulfated chitosans were assessed as inhibitors of HL-60 leukocyte binding to P-selectin, by investigating their effect on leukocyte adhesion to CHO cells expressing human P-selectin under static and flow conditions. The results show that the sulfochitosans exhibit inhibitory activity in this general order: heparin > N-sulfated/6-O-sulfated chitosan ≥ 3-O,6-O-sulfated chitosan > 6-O-sulfated chitosan ≫ N-sulfated chitosan. This suggests that the sulfation of the double site in chitosan is essential for efficient inhibition of P-selectin-mediated HL-60 leukocyte adhesion and that such sulfochitosans may have potential as therapeutic agents against inflammatory disease.

Abbreviations

AC1.2:

a non-blocking mAb against P-selectin

DMF:

dimethylformamide

FBS:

fetal bovine serum

HPLC:

high performance liquid chromatography

N-S-C:

N-sulfated chitosan

N,6-S-C:

N-sulfated/6-O-sulfated chitosan

P-Fc:

recombinant human P-selectin/Fc chimera protei

3,6-S-C:

3-O,6-Osulfated chitosan

6-S-C:

6-O-sulfated chitosan

9E1:

a leukocyte adhesion blocking IgG mAb against P-selectin

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Authors and Affiliations

  1. Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun, 130024, P. R. China

    Jinfeng Huang, Ruifei Wang, Ximing Liu, Xianlu Zeng & Min Wei

Authors
  1. Jinfeng Huang
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  2. Ruifei Wang
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  3. Ximing Liu
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  4. Xianlu Zeng
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  5. Min Wei
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Correspondence to Xianlu Zeng or Min Wei.

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Huang, J., Wang, R., Liu, X. et al. Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions. Cell Mol Biol Lett 18, 200–208 (2013). https://doi.org/10.2478/s11658-013-0084-1

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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