- Mini review
Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications
Cellular & Molecular Biology Letters volume 18, pages 479–493 (2013)
The stromal-vascular cell fraction (SVF) of adipose tissue can be an abundant source of both multipotent and pluripotent stem cells, known as adipose-derived stem cells or adipose tissue-derived stromal cells (ADSCs). The SVF also contains vascular cells, targeted progenitor cells, and preadipocytes. Stromal cells isolated from adipose tissue express common surface antigens, show the ability to adhere to plastic, and produce forms that resemble fibroblasts. They are characterized by a high proliferation potential and the ability to differentiate into cells of meso-, ecto- and endodermal origin. Although stem cells obtained from an adult organism have smaller capabilities for differentiation in comparison to embryonic and induced pluripotent stem cells (iPSs), the cost of obtaining them is significantly lower. The 40 years of research that mainly focused on the potential of bone marrow stem cells (BMSCs) revealed a number of negative factors: the painful sampling procedure, frequent complications, and small cell yield. The number of stem cells in adipose tissue is relatively large, and obtaining them is less invasive. Sampling through simple procedures such as liposuction performed under local anesthesia is less painful, ensuring patient comfort. The isolated cells are easily grown in culture, and they retain their properties over many passages. That is why adipose tissue has recently been treated as an attractive alternative source of stem cells. Essential aspects of ADSC biology and their use in regenerative medicine will be analyzed in this article.
adipose-derived stem cells or adipose tissue-derived stromal cells
adult stem cells
brown adipose tissue
Becker muscular dystrophy
bone marrow stem cells
cluster of differentiation
fibroblast colony-forming unit
Duchenne muscular dystrophy
embryonic stem cells
fetal stem cells
hepatocyte growth factor
hematopoietic stem cells
- Isl 1:
induced pluripotent stem cells
leukemia inhibitory factor
major histocompatibility complex
mesenchymal stem cells
- NGN 3:
- Pax 4:
paired box gene
- PDX 1:
pancreatic duodenal homeobox
- PGE2 :
stromal cell-derived factor
stromal-vascular cell fraction
transforming growth factor-β
transforming growth factor-beta 1
vascular endothelial growth factor
white adipose tissue
Bajek, A., Olkowska, J. and Drewa, T. Mesenchymal stem cells as a therapeutic tool in tissue and organ regeneration. Postepy Hig. Med. Dosw. 65 (2011) 124–132.
Banaś, A. Stem cells — perspectives and dangers. Prz. Med. Uniw. Rzesz. 8 (2010) 117–127.
Skalska, U. and Kontny, E. Regenerative and immunomodulatory properties of adipose-derived mesenchymal stem cells. Post. Biol. Kom. 38 (2011) 363–378.
Lindroos, B., Suuronen, R. and Miettinen, S. The Potential of adipose stem cells in regenerative medicine. Stem Cell Rev. 7 (2011) 269–291.
Jezierska-Woźniak, K., Nosarzewska, D., Tutas, A., Mikołajczyk, A., Okliński, M. and Jurkowski, M.K. Use of adipose tissue as a source of mesenchymal stem cells. Postepy Hig. Med. Dosw. 64 (2010) 326–332.
Zuk, P.A. The Adipose-derived stem cell: looking back and looking ahead. Mol. Biol. Cell 21 (2010) 1783–1787.
Sun, L., Akiyama, K., Zhang, H., Yamaza, T., Hou, Y., Zhao, S., Xu, T., Le, A. and Shi, S. Mesenchymal stem cell transplantation reverses multiorgan dysfunction in systemic lupus erythematosus mice and humans. Stem Cells 27 (2009) 1421–1432.
Cawthorn, W.P., Scheller, E.L. and MacDougald, O.A. Adipose tissue stem cells: the great WAT hope. Trends Endocrinol. Metab. 23 (2012) 270–277.
Zuk, P.A., Zhu, M., Mizuno, H., Huang, J., Futrell, J.W., Katz, A.J., Benhaim, P., Lorenz, H.P. and Hedrick, M.H. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7 (2001) 211–228.
Fraser, J.K., Zhu, M., Wulur, I. and Alfonso, Z. Adipose-derived stem cells. Methods Mol. Biol. 449 (2008) 59–67.
Banaś, A., Teratani, T., Yamamoto, Y., Tokuhara, M., Takeshita, F., Quinn, G., Okochi, H. and Ochiya, T. Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes. Hepatology 46 (2007) 219–228.
Baer, P.C. and Geiger, H. Adipose-derived mesenchymal stromal/stem cells: tissue localization, characterization, and heterogeneity. Stem Cells Int. Article ID 812693, (2012). DOI: 10.1155/2012/812693.
Im, G.-I., Shin, Y.-W. and Lee, K.-B. Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Osteoarthr. Cartil. 13 (2005) 845–853.
Schaffler, A. and Buchler C. Concise review: adipose tissue-derived stromal cells-basic and clinical implications for novel cell-based therapies. Stem Cells 25 (2007) 818–827.
Witkowska-Zimny, M. and Walenko, K. Stem cells from adipose tissue. Cell. Mol. Biol. Lett. 16 (2011) 236–257.
Olkowska-Truchanowicz, J. Isolation and characterization of adipose tissuederived stem cells. Post. Biol. Kom. 35 (2008) 517–526.
Romanov, Y.A., Darevskaya, A.N., Merzlikina, N.V. and Buravkova, L.B. Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization and differentiation potentialities. Bull. Exp. Biol. Med. 3 (2005) 138–143.
Wu, C.-H., Lee, F.-K., Kumar, S.S., Ling, Q.-D., Chang, Y., Chang, Y., Wang, H.-Ch., Chen, H., Chen, D.-Ch., Hsu, S.-T. and Higuchi, A. The isolation and differentiation of human adipose-derived stem cells using membrane filtration. Biomaterials 33 (2012) 8228–8239.
Yang, X., Gong, P., Lin, Y., Zhang, L., Li, X., Yuan, Q., Tan, Z., Wang, Y., Man, Y. and Tang, H. Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway. Arch. Med. Sci. 6 (2010) 152–159.
Olkowska-Truchanowicz, J. Differentiation of adipose tissue-derived stem cells- novel possibilities for tissue engineering and cell-based therapy. Post. Biol. Kom. 36 (2009) 217–231.
Palpant, N.J. and Metzger, J.M. Aesthetic cardiology: adipose-derived stem cells for myocardial repair. Curr. Stem Cell Res. Ther. 5 (2010) 145–152.
Mazo, M., Gavira, J.J., Pelacho, B. and Prosper, F. Adipose-derived stem cells for myocardial infarction. J. Cardiovasc. Transl. Res. 4 (2011) 145–153.
Yamada, Y., Wang, X.-D., Yokayama, S., Fukuda, N. and Takakura, N. Cardiac progenitor cells in brown adipose tissue repaired damaged myocardium. Biochem. Biophys. Res. Commun. 342 (2006) 662–670.
Villiers, J.A., Houreld, N. and Abrahamse, H. Adipose derived stem cells and smooth muscle cells: implications for regenerative medicine. Stem Cell Rev. 5 (2009) 256–265.
Wang, B., Han, J., Gao, Y., Xiao, Z., Chen, B. and Wang, X. The differentiation of rat adipose-derived stem cells into OEC-like cells on collagen scaffolds by co-culturing with OECs. Neurosci. Lett. 421 (2007) 191–196.
Bae, J., Carter, J.E. and Jin, H.K. Adipose tissue-derived stem cells rescue Purkinje neurons and alleviate inflammatory responses in Niemann-Pick disease type C mice. Cell Tissue Res. 340 (2010) 357–369.
Vieira, N.M., Brandalise, V., Zucconi, E., Jazedje, T., Secco, M., Nunes, V.A., Strauss, B.E., Vainzof, M. and Zatz, M. Human multipotent adiposederived stem cells restore dystrophin expression of Duchenne skeletalmuscle cells in vitro. Biol. Cell 100 (2008) 231–241.
Gimble, J.M., Katz, A.J. and Bunnell, B.A. Adipose-derived stem cells for regenerative medicine. Circ. Res. 100 (2007) 1249–1260.
Okura, H., Komoda, H., Fumimoto, Y., Lee, C.M., Nishida, T., Sawa, Y. and Matsuyama, A. Transdifferentiation of human adipose tissue-derived stromal cells into insulin-producing clusters. J. Artif. Organs. 12 (2009) 123–130.
Silva, A.C., Percegona, L.S., Franca, A.L., dos Santos, T.M., Perini, C.C., Gonzalez, P., Rebelatto, C.L.K., Camara, N.O.S. and Aita, C.A.M. Expression of pancreatic endocrine markers by mesenchymal stem cells from human adipose tissue. Transplant. Proc. 44 (2012) 2495–2496.
Marappagounder, D., Somasundaram, I., Dorairaj, S. and Sankaran R.J. Differentiation of mesenchymal stem cells derived from human bone marrow and subcutaneous adipose tissue into pancreatic islet-like clusters in vitro. Cell. Mol. Biol. Lett. 18 (2013) 75–88.
Sterodimas, A., de Faria, J., Nicaretta, B. and Pitanguy, I. Tissue engineering with adipose-derived stem cells (ADSCs): Current and future applications. J. Plast. Reconstr. Aesthet. Surg. 63 (2010) 1886–1892.
Muehlberg, F.L., Song, Y.-H., Krohn, A., Pinilla, S.P., Droll, L.H., Leng, X., Seidensticker, M., Ricke, J., Altman, A.M., Devarajan, E., Liu, W., Arlinghaus, R.B. and Alt, E.U. Tissue-resident stem cells promote breast cancer growth and metastasis. Carcinogenesis 30 (2009) 589–597.
Cousin, B., Ravet, E., Poglio, S., de Toni, F., Bertuzzi, M., Lulka, H., Touil, I., André, M., Grolleau, J.L., Péron, J.M., Chavoin, J.P., Bourin, P., Pénicaud, L., Casteilla, L., Buscail, L., and Cordelier, P. Adult stromal cells derived from human adipose tissue provoke pancreatic cancer cell death both in vitro and in vivo. Plos One 7 (2009) e6278. DOI:10.1371/journal.pone.0006278.
Kucerova, L., Altanerova, V., Matuskova, M., Tyciakova, S., and Altaner, C. Adipose tissue-derived human mesenchymal stem cells mediated prodrug cancer gene therapy. Cancer Res. 67 (2007) 6304–6313.
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Harasymiak-Krzyżanowska, I., Niedojadło, A., Karwat, J. et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett 18, 479–493 (2013). https://doi.org/10.2478/s11658-013-0101-4
- Mesenchymal stem cells
- Regenerative medicine
- Adipose tissue
- Stem cell therapy
- Adipose-derived stem cells
- Stromal cells
- Flow cytometry