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The expression of the eotaxins IL-6 and CXCL8 in human epithelial cells from various levels of the respiratory tract

Cellular & Molecular Biology Letters volume 18, pages 612–630 (2013)Cite this article

Abstract

Airway epithelium acts as multifunctional site of response in the respiratory tract. Epithelial activity plays an important part in the pathophysiology of obstructive lung disease. In this study, we compare normal human epithelial cells from various levels of the respiratory tract in terms of their reactivity to pro-allergic and pro-inflammatory stimulation. Normal human nasal, bronchial and small airway epithelial cells were stimulated with IL-4 and IL-13. The expressions of the eotaxins IL-6 and CXCL8 were evaluated at the mRNA and protein levels. The effects of pre-treatment with IFN-γ on the cell reactivity were measured, and the responses to TNF-α, LPS and IFN-γ were evaluated. All of the studied primary cells expressed CCL26, IL-6 and IL-8 after IL-4 or IL-13 stimulation. IFN-γ pre-treatment resulted in decreased CCL26 and increased IL-6 expression in the nasal and small airway cells, but this effect was not observed in the bronchial cells. IL-6 and CXCL8 were produced in varying degrees by all of the epithelial primary cells in cultures stimulated with TNF-α, LPS or IFN-γ. We showed that epithelial cells from the various levels of the respiratory tract act in a united way, responding in a similar manner to stimulation with IL-4 and IL-13, showing similar reactivity to TNF-α and LPS, and giving an almost unified response to IFN-γ pre-stimulation.

Abbreviations

ATCC:

American Type Culture Collection

BEGM:

bronchial epithelial cell growth medium

COPD:

chronic obstructive pulmonary disease

IFN-Îł:

interferon Îł

LPS:

lipopolysaccharide

TGF-ÎČ:

transforming growth factor beta

TNF-α:

tumor necrosis factor alpha

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Authors and Affiliations

  1. Department of Internal Medicine, Pneumonology and Allergology, Medical University of Warsaw, Banacha 1a, 02-097, Warsaw, Poland

    Magdalena PapliƄska-Goryca, Patrycja Nejman-Gryz, Ryszarda Chazan & Hanna Grubek-Jaworska

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  1. Magdalena PapliƄska-Goryca
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  2. Patrycja Nejman-Gryz
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Correspondence to Magdalena PapliƄska-Goryca.

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PapliƄska-Goryca, M., Nejman-Gryz, P., Chazan, R. et al. The expression of the eotaxins IL-6 and CXCL8 in human epithelial cells from various levels of the respiratory tract. Cell Mol Biol Lett 18, 612–630 (2013). https://doi.org/10.2478/s11658-013-0107-y

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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