Open Access

The expression of the eotaxins IL-6 and CXCL8 in human epithelial cells from various levels of the respiratory tract

  • Magdalena Paplińska-Goryca1Email author,
  • Patrycja Nejman-Gryz1,
  • Ryszarda Chazan1 and
  • Hanna Grubek-Jaworska1
Cellular & Molecular Biology LettersAn International Journal201318:107

https://doi.org/10.2478/s11658-013-0107-y

Received: 16 July 2013

Accepted: 27 November 2013

Published: 2 December 2013

Abstract

Airway epithelium acts as multifunctional site of response in the respiratory tract. Epithelial activity plays an important part in the pathophysiology of obstructive lung disease. In this study, we compare normal human epithelial cells from various levels of the respiratory tract in terms of their reactivity to pro-allergic and pro-inflammatory stimulation. Normal human nasal, bronchial and small airway epithelial cells were stimulated with IL-4 and IL-13. The expressions of the eotaxins IL-6 and CXCL8 were evaluated at the mRNA and protein levels. The effects of pre-treatment with IFN-γ on the cell reactivity were measured, and the responses to TNF-α, LPS and IFN-γ were evaluated. All of the studied primary cells expressed CCL26, IL-6 and IL-8 after IL-4 or IL-13 stimulation. IFN-γ pre-treatment resulted in decreased CCL26 and increased IL-6 expression in the nasal and small airway cells, but this effect was not observed in the bronchial cells. IL-6 and CXCL8 were produced in varying degrees by all of the epithelial primary cells in cultures stimulated with TNF-α, LPS or IFN-γ. We showed that epithelial cells from the various levels of the respiratory tract act in a united way, responding in a similar manner to stimulation with IL-4 and IL-13, showing similar reactivity to TNF-α and LPS, and giving an almost unified response to IFN-γ pre-stimulation.

Key words

CXCL8 Eotaxin-3 Inflammation Interferon gamma Interleukin 6 Respiratory epithelium United airways

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