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MiR-34a regulates apoptosis in liver cells by targeting the KLF4 gene

Cellular & Molecular Biology Letters volume 19, pages 52–64 (2014)Cite this article

Abstract

MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstrate that miR-34a targets the 3′-untranslated mRNA region of KLF4 and show that overexpression of miR-34a induces a significant level of apoptosis in BNL CL.2 cells exposed to doxorubicin or 10 Gy X-ray. Our data suggest that the effects of miR-34a on apoptosis occur due to the downregulation of KLF4.

Abbreviations

ALDH2:

aldehyde dehydrogenase-2

BCL2:

B-cell lymphoma-2

FCM:

flow cytometry

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

HEK:

human embryonic kidney

KLF4:

Krüppel-like factor 4

TBST:

Tris-buffered saline with Tween

UTR:

untranslated region

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Authors and Affiliations

  1. Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou City, China

    Qiu Chen, Lei Li, Yu Tu, Lu Lin Zheng, Wei Liu, Xue Yong Zuo, Shu Yu Zhang, Wei Zhu, Jian Ping Cao & Feng Mei Cui

  2. Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou City, China

    Yong Ming He

  3. Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai City, China

    Jun Hou

Authors
  1. Qiu Chen
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  2. Lei Li
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  3. Yu Tu
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  5. Wei Liu
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Corresponding authors

Correspondence to Feng Mei Cui or Jun Hou.

Additional information

Qiu Chen and Lei Li contributed equally to this work

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Chen, Q., Li, L., Tu, Y. et al. MiR-34a regulates apoptosis in liver cells by targeting the KLF4 gene. Cell Mol Biol Lett 19, 52–64 (2014). https://doi.org/10.2478/s11658-013-0115-y

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  • DOI: https://doi.org/10.2478/s11658-013-0115-y

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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