Open Access

MiR-34a regulates apoptosis in liver cells by targeting the KLF4 gene

  • Qiu Chen1,
  • Lei Li1,
  • Yu Tu1,
  • Lu Lin Zheng1,
  • Wei Liu1,
  • Xue Yong Zuo1,
  • Yong Ming He2,
  • Shu Yu Zhang1,
  • Wei Zhu1,
  • Jian Ping Cao1,
  • Feng Mei Cui1Email author and
  • Jun Hou3Email author
Cellular & Molecular Biology LettersAn International Journal201419:115

https://doi.org/10.2478/s11658-013-0115-y

Received: 27 June 2013

Published: 10 January 2014

Abstract

MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstrate that miR-34a targets the 3′-untranslated mRNA region of KLF4 and show that overexpression of miR-34a induces a significant level of apoptosis in BNL CL.2 cells exposed to doxorubicin or 10 Gy X-ray. Our data suggest that the effects of miR-34a on apoptosis occur due to the downregulation of KLF4.

Key words

MiR-34a KLF4 Apoptosis Liver Irradiation Doxorubicin

Notes

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