Open Access

Study on the activity of the signaling pathways regulating hepatocytes from G0 phase into G1 phase during rat liver regeneration

  • Menghua Li1, 2,
  • Xiaochun Zhou1, 2,
  • Jinxin Mei1, 2,
  • Xiaofang Geng1, 2,
  • Yun Zhou1, 2,
  • Weimin Zhang2 and
  • Cunshuan Xu1, 2Email author
Cellular & Molecular Biology LettersAn International Journal201419:188

https://doi.org/10.2478/s11658-014-0188-2

Received: 20 November 2013

Accepted: 4 March 2014

Published: 19 March 2014

Abstract

Under normal physiological conditions, the majority of hepatocytes are in the functional state (G0 phase). After injury or liver partial hepatectomy (PH), hepatocytes are rapidly activated to divide. To understand the mechanism underlying hepatocyte G0/G1 transition during rat liver regeneration, we used the Rat Genome 230 2.0 Array to determine the expression changes of genes, then searched the GO and NCBI databases for genes associated with the G0/G1 transition, and QIAGEN and KEGG databases for the G0/G1 transition signaling pathways. We used expression profile function (E t ) to calculate the activity level of the known G0/G1 transition signal pathways, and Ingenuity Pathway Analysis 9.0 (IPA) to determine the interactions among these signaling pathways. The results of our study show that the activity of the signaling pathways of HGF, IL-10 mediated by p38MAPK, IL-6 mediated by STAT3, and JAK/STAT mediated by Ras/ERK and STAT3 are significantly increased during the priming phase (2–6 h after PH) of rat liver regeneration. This leads us to conclude that during rat liver regeneration, the HGF, IL-10, IL-6 and JAK/STAT signaling pathways play a major role in promoting hepatocyte G0/G1 transition in the regenerating liver.

Keywords

Rat liver regenerationSignal transductionHepatocyte G0/G1 transitionHGFIL-10IL-6JAK/STATp38MAPKRas/ERKSTAT3Ingenuity pathway analysis 9.0 (IPA)

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