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The influence of heterocyclic compound-PAMAM dendrimer complexes on evoked electrical responses in slices of hypoxic brain tissue

Cellular & Molecular Biology Letters volume 19, pages 243–248 (2014)Cite this article

Abstract

We used complexes between a fourth generation polyamidoamine (PAMAM) dendrimer and one of two heterocyclic compounds — 1-(6-hydroxyhexyl)-3-(5-phenyl-isoxazole-3-yl)-urea or 5-phenyl-isoxazole-3-carboxylic acid — to reduce oxygen consumption in transverse slices of the hippocampus taken from 4-week old male rats. In vitro electrophysiological experiments revealed that the inhibitory effect of the hypoxic state on the evoked responses was enhanced in the presence of the complexes. The data were analyzed in terms of the potential antitumor effects of these complexes.

Abbreviations

ACSF:

artificial cerebrospinal fluid

EPSP:

excitatory postsynaptic potential

PAMAM:

polyamidoamine

PS:

population spike

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Author information

Affiliations

  1. Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus

    Vladimir I. Potkin, Sergey K. Petkevich & Alexey V. Kletskov

  2. Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, Minsk, Belarus

    Dzmitry Shcharbin

  3. Institute of Physiology of National Academy of Sciences of Belarus, Minsk, Belarus

    Andrey A. Denisov, Svetlana G. Paschkevich, Vladimir V. Kazbanov, Tatiana A. Gurinovich & Vladimir A. Kulchitsky

  4. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland

    Maria Bryszewska

  5. Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA

    Dmitri O. Lapotko

  6. Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja str. 27, Minsk, 220072, Belarus

    Dzmitry Shcharbin

Authors
  1. Vladimir I. Potkin
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  2. Dzmitry Shcharbin
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  3. Andrey A. Denisov
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  4. Svetlana G. Paschkevich
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  5. Maria Bryszewska
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  6. Sergey K. Petkevich
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  7. Alexey V. Kletskov
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  8. Dmitri O. Lapotko
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  9. Vladimir V. Kazbanov
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  10. Tatiana A. Gurinovich
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  11. Vladimir A. Kulchitsky
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Corresponding author

Correspondence to Dzmitry Shcharbin.

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Potkin, V.I., Shcharbin, D., Denisov, A.A. et al. The influence of heterocyclic compound-PAMAM dendrimer complexes on evoked electrical responses in slices of hypoxic brain tissue. Cell Mol Biol Lett 19, 243–248 (2014). https://doi.org/10.2478/s11658-014-0193-5

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Keywords

  • Hypoxia
  • Dendrimer
  • Brain slices
  • Postsynaptic potential
  • Antitumor effects
  • Heterocycles
  • Isoxazoles

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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