Open Access

Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering

  • Aleksandra Zuber1,
  • Julia Borowczyk1,
  • Eliza Zimolag1,
  • Malgorzata Krok2,
  • Zbigniew Madeja1,
  • Elzbieta Pamula2 and
  • Justyna Drukala1Email author
Cellular & Molecular Biology LettersAn International Journal201419:197

https://doi.org/10.2478/s11658-014-0197-1

Received: 8 December 2013

Accepted: 5 May 2014

Published: 13 May 2014

Abstract

Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.

Keywords

Burns Skin regeneration Wound healing Keratinocytes Fibroblasts Biomaterials PLGA Tissue engineering

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