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MiR-30b is involved in methylglyoxal-induced epithelial-mesenchymal transition of peritoneal mesothelial cells in rats


Epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMC) is a major contributor to the pathogenesis of peritoneal fibrosis. EMT is at least in part caused by repeated exposure to glucose degradation products (GDPs), such as methylglyoxal (MGO). MiRNA contributes greatly to the EMT of PMCs. In this study, we tried to profile whether differences exist between the peritoneal membrane (PM) miRNA expression seen in control rats and that seen in rats injected intraperitoneally with MGO. We assessed whether miR-30b has a possible role in MGO-induced EMT of PMCs in rats. Comparative miRNA expression array and real-time PCR analyses were conducted for the control group at the start of the experiment and for the MGO group after 1 and 2 weeks. During the second week, the MGO rats were treated with: a chemically modified antisense RNA oligonucleotide (ASO) complementary to the mature miR-30b (ASO group); an miR-30b mismatch control sequence (MIS group); or a citrate buffer (EMT group). Bioinformatic analyses indicated that the 3′ untranslated region (3′-UTR) of bone morphogenetic protein 7 (BMP7) mRNA did contain a putative binding site for miR-30b. We also tried to investigate whether miR-30b targeted BMP7 in vitro by transfection. Of the upregulated miRNAs, miR-30b expression demonstrated the greatest increase. The administration of miR-30b ASO for two weeks significantly reduced α-SMA excretion and upregulated E-cadherin and BMP-7 expression. Our in vitro study showed that miR-30b directly targeted and inhibited BMP7 by binding to its 3’-UTR. Our results revealed that miR-30b is involved in MGO-induced EMT of PMCs in rats.



3′ untranslated region


α-smooth muscle actin


antisense RNA oligonucleotide


bone morphogenetic protein 7


ethylene diamine tetraacetic acid


epithelial-mesenchymal transition


endstage renal disease


glucose degradation products


horseradish peroxidase




peritoneal dialysis


peritoneal dialysis fluids


peritoneal membrane


peritoneal mesothelial cells


rat peritoneal mesothelial cells


renal replacement therapy


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Correspondence to Da Tian.

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Liu, H., Zhang, N. & Tian, D. MiR-30b is involved in methylglyoxal-induced epithelial-mesenchymal transition of peritoneal mesothelial cells in rats. Cell Mol Biol Lett 19, 315–329 (2014).

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  • Methylglyoxal
  • miR-30b
  • Epithelial-mesenchymal transition
  • Peritoneal dialysis
  • Rats
  • Peritoneal fibrosis
  • MicroRNA
  • Bone morphogenetic protein 7
  • E-cadherin
  • Peritoneal membrane