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The lectin-binding pattern of nucleolin and its interaction with endogenous galectin-3

Cellular & Molecular Biology Letters volume 19, pages 461–482 (2014)Cite this article

Abstract

Unlike nuclear nucleolin, surface-expressed and cytoplasmic nucleolin exhibit Tn antigen. Here, we show localization-dependent differences in the glycosylation and proteolysis patterns of nucleolin. Our results provide evidence for different paths of nucleolin proteolysis in the nucleus, in the cytoplasm, and on the cell surface. We found that full-length nucleolin and some proteolytic fragments coexist within live cells and are not solely the result of the preparation procedure. Extranuclear nucleolin undergoes N- and O-glycosylation, and unlike cytoplasmic nucleolin, membrane-associated nucleolin is not fucosylated. Here, we show for the first time that nucleolin and endogenous galectin-3 exist in the same complexes in the nucleolus, the cytoplasm, and on the cell surface of melanoma cells. Assessments of the interaction of nucleolin with galectin-3 revealed nucleolar co-localization in interphase, suggesting that galectin-3 may be involved in DNA organization and ribosome biogenesis.

Abbreviations

AAA:

Aleuria aurantia agglutinin

DSA:

Datura stramonium gglutinin

GNA:

Galanthus nivalis agglutinin

H antigen:

Fucα1-2Galβ1-4GlcNAcβ1-3Gal-

LEA:

Lycopersicon esculentum agglutinin

Lex:

Galβ1-4(Fucα1-3)GlcNAcβ-

LTA:

Tetragonolobus purpureus agglutinin

MAA-II:

Maackia amurensis agglutinin

SNA-I:

Sambucus nigra agglutinin

PHA-E:

Phaseolus vulgaris erythroagglutinin

PHAL:

Phaseolus vulgaris leucoagglutinin

PNA:

peanut agglutinin

T antigen:

Galβ1-3GalNAcα1 -Ser/Thr

Tn antigen:

GalNAcα1 -Ser/Thr

UEA-I:

Ulex europaeus agglutinin

VVA:

Vicia villosa agglutinin

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Authors and Affiliations

  1. Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, 9 Gronostajowa Street, 30-387, Kraków, Poland

    Dorota Hoja-Łukowicz & Anna Lityńska

  2. Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Street, 30-387, Kraków, Poland

    Sylwia Kedracka-Krok

  3. Malopolska Centre of Biotechnology, Jagiellonian University, 7 Gronostajowa Street, 30-387, Kraków, Poland

    Sylwia Kedracka-Krok

  4. Departament of Neurophysiology, Laboratory of Neuropsychology, Nencki Institute of Experimental Biology Polish Academy of Sciences, 3 Pasteur Street, 02-093, Warszawa, Poland

    Weronika Duda

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  1. Dorota Hoja-Łukowicz
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  3. Weronika Duda
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Correspondence to Dorota Hoja-Łukowicz.

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Hoja-Łukowicz, D., Kedracka-Krok, S., Duda, W. et al. The lectin-binding pattern of nucleolin and its interaction with endogenous galectin-3. Cell Mol Biol Lett 19, 461–482 (2014). https://doi.org/10.2478/s11658-014-0206-4

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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