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Promoted megakaryocytic differentiation of K562 cells through oxidative stress caused by near ultraviolet irradiation
Cellular & Molecular Biology Letters volume 19, pages 590–600 (2014)
Reactive oxygen species (ROS) have been proven to be important activators for various cellular activities, including cell differentiation. Several reports showed the necessity of ROS during cell differentiation of the megakaryocytic (MK) lineage. In this study, we employed near ultraviolet (near-UV) irradiation to generate endogenous oxidative stress in an MK differentiation process of K562 cells with phorbol 12-myristate 13-acetate (PMA) induction. A significant increase in the intracellular ROS level was detected on day 1 after near-UV irradiation. In the initial stage of differentiation, a shifted fraction of G1 and G2 phase cells was obtained using near-UV irradiation, giving an increased percentage of G2 phase cells (up from 31.1 to 68.7%). The near-UV irradiation-induced upregulation of the p21 gene, which is a cell cycle inhibitor, suggested that the G2 phase cells were prevented from undergoing cell division. It was found that the percentage of high ploidy (8N and 16N) cells was enhanced significantly at the later stage of the K562 cell culture with near-UV irradiation. Moreover, time-lapse analysis showed that near-UV irradiation encouraged the expression of CD41, a specific surface marker of megakaryocytes. This is the first report that the elevated oxidative stress through the near-UV irradiation promoted the MK differentiation of PMA-induced K562 cells.
ethylene diamine tetra-acetic acid
phorbol 12-myristate 13-acetate
reactive oxygen species
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Nurhayati, R.W., Ojima, Y., Nomura, N. et al. Promoted megakaryocytic differentiation of K562 cells through oxidative stress caused by near ultraviolet irradiation. Cell Mol Biol Lett 19, 590–600 (2014). https://doi.org/10.2478/s11658-014-0215-3
- Near ultraviolet
- K562 cells
- Megakaryocytic differentiation
- Reactive oxygen species
- Cell cycle
- Phorbol 12-myristate 13-acetate