- Research Article
Rapid proliferation of activated lymph node CD4+ T cells is achieved by greatly curtailing the duration of gap phases in cell cycle progression
Cellular & Molecular Biology Letters volume 19, pages 638–648 (2014)
Peripheral T cells are in G0 phase and do not proliferate. When they encounter an antigen, they enter the cell cycle and proliferate in order to initiate an active immune response. Here, we have determined the first two cell cycle times of a leading population of CD4+ T cells stimulated by PMA plus ionomycin in vitro. The first cell cycle began around 10 h after stimulation and took approximately 16 h. Surprisingly, the second cell cycle was extremely rapid and required only 6 h. T cells might have a unique regulatory mechanism to compensate for the shortage of the gap phases in cell cycle progression. This unique feature might be a basis for a quick immune response against pathogens, as it maximizes the rate of proliferation.
carboxyfluorescein diacetate succinimidyl ester
fetal bovine serum
major histocompatibility complex
protein kinase C
phorbol 12-myristate 13-acetate
T cell receptor
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These authors contributed equally to this work
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Mishima, T., Toda, S., Ando, Y. et al. Rapid proliferation of activated lymph node CD4+ T cells is achieved by greatly curtailing the duration of gap phases in cell cycle progression. Cell Mol Biol Lett 19, 638–648 (2014). https://doi.org/10.2478/s11658-014-0219-z