Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs11658-018-0079-z/MediaObjects/11658_2018_79_Fig3_HTML.gif)
PlasDIC images of HeLa cells demonstrating the morphological changes induced by ESE-16. a and b Cells propagated in growth medium (a) and vehicle-treated control cells (b) were confluent, with majority of cells in interphase. c ESE-16-treated cells: several cells are blocked in metaphase and there are visible apoptotic characteristics such as shrunken cells and apoptotic bodies. d Hallmarks of apoptosis were observed in the positive control cells, which were exposed to actinomycin D (20× magnification)