Fig. 4

Light microscopy images revealing the morphological effects of ESE-16 on the nuclear and cytoplasmic structures in HeLa cells. a and b Cells propagated in growth medium (a) and vehicle-treated control cells (b) showed a dense population and normal division. Most cells were found to be in interphase. c and d Cells exposed to ESE-16 (c) and 0.1 μg/ml actinomycin D (d) revealed membrane blebbing, apoptotic bodies and an increased number of metaphase cells after 24 h. Both treatments resulted in a compromised cell density (40× magnification)