Fig. 1

Expression levels of miR-219-5p were downregulated in ESCC tissues and cell lines. a A comparison of miR-219-5p expression levels between 20 paired ESCC tissues (P1–P20) and corresponding non-tumor (NT) tissues. MiR-219-5p expression was normalized to U6, calculated using the 2^-ΔΔCt method, and then compared with miR-219-5p expression in NT tissues. b Quantitative analysis of miR-219-5p levels determined using real-time PCR in four ESCC cell lines (ECA109, KYSE150, EC9706, TE-9) and one normal esophageal cell line (Het-1A). The data are presented as the means ± standard deviations. Experiments were performed in triplicate and repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001; miR, microRNA; ESCC, esophageal squamous cell carcinoma