Fig. 6

Knockdown of CCNA2 potentiated the anti-tumor effects of miR-219-5p. EC9706 cells were transfected with si-CCNA2, miR-219-5p or co-transfected with miR-219-5p and si-CCNA2. a Real-time PCR was used to determine the expression of CCNA2 in EC9706 cells. b Cell proliferation was measured using the MTT assay over five consecutive days. c The percentage of cells in the G0/G1, S and G2/M phases was analyzed using flow cytometry. The data are reported as means ± SD of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, compared with si-NC, si-CCNA2 or miR-219-5p