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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5

Fig. 6

Effect of asiaticoside on Nrf2 activation in HPMCs. Notes: (A) After asiaticoside (150 μM) treatment for various times (0, 1, 3, 6, 12, 24 h), the expression of Nrf2 in the nucleus and HO-1 expression were detected by immunoblotting. Histone H3 and β-actin were used as the loading controls for the nucleus and cytosol, respectively. (B) After treatment with various doses of asiaticoside (0, 50, 100, 150 μM) for 6 h, Nrf2 and HO-1 expression were detected by immunoblotting. (C) After treated with asiaticoside at various concentrations (0, 50, 100, 150 μM) and TGF-β1 (10 ng/cm3) treatment for 6 h, Nrf2 and HO-1 expression were detected by immunoblotting. β-actin was used as a loading control. Densitometric analysis of the expression of Nrf2 and HO-1 are shown as unique figures. Data are expressed as the mean ± SEM, *p < 0.05 vs. control, **p < 0.01 vs. control; #p < 0.05 vs. TGF-β1 treatment; ##p < 0.01 vs. TGF-β1 treatment. (D) Immunofluorescence assays were performed to observe the subcellular translocation of Nrf2. Nrf2 expression in the membrane, cytoplasm and nucleus were visualized based on the green fluorescent signal obtained by fluorescence microscopy (scale bar = 50 μm). Abbreviations: AS, asiaticoside; TGF-β1, transforming growth factor-β1; Nrf2, nuclear factor erythroid-2-related factor 2; HO-1, heme oxygenase 1

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