Fig. 2
From: Mitochondrial potassium channels: A novel calcitriol target
Effect of calcitriol on large-conductance calcium-activated potassium channel from mitochondria isolated from human glioblastoma cells. A Recordings at + 40 mV in symmetrical (150/150 mM) KCl solutions with high calcium ion concentration (100 μM Ca2+); after application of 100 nM calcitriol (1 and 5 min after application), and after wash-out solution with calcitriol. (o1) (o2) indicates an open state of channels 1 and 2 respectively. (c) indicates a closed state. Recordings are from one representative out of three independent experiments. B Bar graphs summarizing the effect of calcitriol on the open probability (Po) of the mitoBKCa channel and the current amplitude (pA) in the presence of 100 μM calcium. The upper panel shows changes of the Po after adding increasing concentrations of calcitriol and wash-out (n = 3). The lower panel shows changes of the current after adding increasing concentrations of calcitriol and wash-out (n = 3). C Recordings at + 40 mV in symmetrical (150/150 mM) KCl solutions with low calcium ion concentration (10 μM Ca2+, control), after application of 100 nM and 300 nM calcitriol and wash-out. (o1) (o2) indicates an open state of channels 1 and 2 respectively. (c) indicates a closed state. Recordings are from one representative out of three independent experiments. D Bar graphs summarizing the effect of 100 nM and 300 nM calcitriol and wash-out on the open probability (Po) of the mitoBKCa channel and the current amplitude (pA) in 10 μM Ca2+ ion concentration. The left panel shows changes of the Po after adding 100 nM or 300 nM calcitriol and wash-out (n = 3). The right panel shows changes in the current after adding 100 nM or 300 nM calcitriol and wash-out (n = 3)