Fig. 7

Detection of miR-874-3p expression and its targeting to necroptosis. A Quantitative PCR detects the expression of miR-874-3p in each group in vivo. **p < 0.01, compared with sham group; ^##p < 0.01, compared with UUO group. B Detection of miR-874-3p targeting RIPK1 by dual-luciferase reporter gene assay. **p < 0.01, compared with WT + NC group. C Western blot of necroptosis-related markers (RIPK1, RIPK3, MLKL, p-MLKL, and PGAM5) in each group. D–G Relative expression of necroptosis-related markers detected by western blot. *p < 0.05, **p < 0.01, compared with control group; ^#p < 0.05, ^##p < 0.01, compared with cisplatin (Cis) group. H Western blot of Mfn1 and Mfn2 in each group. I, J Relative expression of Mfn1 and Mfn2 detected by western blot. *p < 0.05, **p < 0.01, compared with control group; ^#p < 0.05, ^##p < 0.01, compared with Cis group