Fig. 1

N6-methyladenosine lncRNAs in GCSCs. A Percentage of tumorsphere formation of GCSCs and GCNSCs. B Tumorsphere formation assay of a single cell from GCSCs; C detection of the expressions of stemness genes in GCSCs and GCNSCs with Western blot. D Efficiency of the migration of GCSCs. GCSCs and GCNSCs were subjected to Transwell migration assays to examine the cell migration. The representative images of the migrated cells are shown on the left. The percentage of the migrated cells is indicated on the right (**, p < 0.01). Scale bar, 50 μm. (E) Chemoresistance capacity of GCSCs. GCSCs or GCNSCs were treated with different concentrations of cisplatin. At 48 h after treatment, the cells were subjected to cell viability assays (**, p < 0.01). The half maximal inhibitory concentration (IC₅₀) values were calculated. F The m6A modification of RNAs in GCSCs and GNCSCs. G Heatmap of the differentially expressed m6A-modified lncRNAs in GCSCs and GNCSCs. H Expression profiles of methylated lncRNAs in GCNSCs and GCSCs. **, p < 0.01