Fig. 5
From: Methylated lncRNAs suppress apoptosis of gastric cancer stem cells via the lncRNA–miRNA/protein axis
Underlying mechanism of lncRNAs in GCSCs. A The relative enrichment of miRNAs interacted with lncRNAs in GCSCs. B Dual-luciferase reporter assay for potential miRNAs-lncRNAs interaction in GCSCs. C The expression profiles of miR-411-3p and miR-24-3p in GCSCs and GCNSCs. D Prediction of potential target genes of miRNAs using microT, miRmap, and TargetScan. E Validation for overexpression of miRNAs in GCSCs. F Detection of the expression levels of target genes in the miRNA-overexpressed GCSCs. G Validation of interaction between miRNAs and target genes by dual-luciferase reporter assay. H The expression levels of target genes of miRNAs in GCSCs and GCNSCs. I Western blot for target genes of miRNAs in GCSCs and GCNSCs. J Caspase-3/7 assay for AP1G1 or SERTAD1 silencing GCSCs. K Western blot for stemness genes in AP1G1 or SERTAD1 silencing GCSCs. L The expression level of AP1G1 or SERTAD1 in PSMA3-AS1 or MIR22HG silencing GCSCs. M Western blot analysis for target genes of miRNAs in PSMA3-AS1 or MIR22HG-silenced GCSCs. **, p < 0.01