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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: Methylated lncRNAs suppress apoptosis of gastric cancer stem cells via the lncRNA–miRNA/protein axis

Fig. 6

Interactions between m6A-modified lncRNAs and proteins. A SDS-PAGE for lncRNAs pulldown assay. The arrows indicate the proteins identified. M, protein marker. B Western blot analysis of the proteins bound to lncRNAs. C Detection of the stability of proteins bound to lncRNAs in PSMA3-AS1 or MIR22HG silencing GCSCs. D The levels of mRNAs and proteins of EEF1A1 and LRPPRC in GCSCs. E Validation of silencing of EEF1A1 or LRPPRC in GCSCs by RT-qPCR and Western blot assay. F Cell viability for EEF1A1 or LRPPRC silencing GCSCs. G Cell cycle analysis of EEF1A1 or LRPPRC silencing GCSCs. H Caspase-3/7 assay of EEF1A1 or LRPPRC silencing GCSCs. I Western blot for stemness genes of EEF1A1 or LRPPRC silencing GCSCs. **, p < 0.01

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