Articles

Heat shock protein 27 plays a protective role in thoracic aortic dissection by promoting cell proliferation and inhibiting apoptosis

Thoracic aortic dissection (TAD) is one of the most severe aortic diseases. The study aimed to explore the potential role of heat shock protein 27 (HSP27) in the pathogenesis of TAD using an in vitro model of ...

Authors: Sili Zou, Mingfang Liao, Junlin Yang, Tong Huang, Mark Green, Jianjin Wu and Lefeng Qu

miR-200b downregulates CFTR during hypoxia in human lung epithelial cells

Hypoxic conditions induce the expression of hypoxia-inducible factors (HIFs) that allow cells to adapt to the changing conditions and alter the expression of a number of genes including the cystic fibrosis tra...

Authors: Sylwia Bartoszewska, Wojciech Kamysz, Bogdan Jakiela, Marek Sanak, Jarosław Króliczewski, Zsuzsa Bebok, Rafal Bartoszewski and James F. Collawn

TGF-β1-induced epithelial–mesenchymal transition in lung cancer cells involves upregulation of miR-9 and downregulation of its target, E-cadherin

TGF-β1 plays an important role in the epithelial–mesenchymal transition (EMT) of epithelial cancers, including non-small cell lung cancer (NSCLC). While the full underlying mechanism remains unclear, miR-9 is ...

Authors: Hui Wang, Qian Wu, Ying Zhang, Hua-Nan Zhang, Yong-Bin Wang and Wei Wang

The novel protein C3orf43 accelerates hepatocyte proliferation

Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicate...

Authors: Chunyan Zhang, Cuifang Chang, Deming Li, Fuchun Zhang and Cunshuan Xu

Are online prediction tools a valid alternative to genomic profiling in the context of systemic treatment of ER-positive breast cancer?

Clinicians use clinical and pathological parameters, such as tumour size, grade and nodal status, to make decisions on adjuvant treatments for breast cancer. However, therapeutic decisions based on these featu...

Authors: Umar Wazir, Kinan Mokbel, Amtul Carmichael and Kefah Mokbel

Luteolin, quercetin, genistein and quercetagetin inhibit the effects of lipopolysaccharide obtained from Porphyromonas gingivalis in H9c2 cardiomyoblasts

One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is Porphyromonas gingivalis. It is a Gram-negative bacteria harvested from chronic periodontit...

Authors: Gloria Gutiérrez-Venegas, Alfredo Torras-Ceballos, Juan Arturo Gómez-Mora and Berenice Fernández-Rojas

Gene functioning and storage within a folded genome

In mammals, genomic DNA that is roughly 2 m long is folded to fit the size of the cell nucleus that has a diameter of about 10 μm. The folding of genomic DNA is mediated via assembly of DNA-protein complex, ch...

Authors: Sergey V. Razin and Sergey V. Ulianov

PIWI-like protein, HIWI2 is aberrantly expressed in retinoblastoma cells and affects cell-cycle potentially through OTX2

Retinoblastoma (RB), a childhood cancer, is caused by biallelic mutation of the RB1 gene, but its development is not clearly understood. Furthermore, the presence of a cancer stem cell subpopulation in RB might i...

Authors: Suganya Sivagurunathan, Jayamuruga Pandian Arunachalam and Subbulakshmi Chidambaram

ADAM23 promotes neuronal differentiation of human neural progenitor cells

ADAM23 is widely expressed in the embryonic central nervous system and plays an important role in tissue formation.

Authors: Annett Markus-Koch, Oliver Schmitt, Susanne Seemann, Jan Lukas, Dirk Koczan, Mathias Ernst, Georg Fuellen, Andreas Wree, Arndt Rolfs and Jiankai Luo

Relevance of the NR4A sub-family of nuclear orphan receptors in trophoblastic BeWo cell differentiation

Nur-77, a member of the NR4A sub-family of nuclear orphan receptors, is downregulated in the placentae of pre-eclamptic women. Here, we investigate the relevance of Nor-1, Nurr-1 and Nur-77 in trophoblastic ce...

Authors: Sudha Saryu Malhotra and Satish Kumar Gupta

SFRP2 enhances the osteogenic differentiation of apical papilla stem cells by antagonizing the canonical WNT pathway

Exploring the molecular mechanisms underlying directed differentiation is helpful in the development of clinical applications of mesenchymal stem cells (MSCs). Our previous study on dental tissue-derived MSCs ...

Authors: Luyuan Jin, Yu Cao, Guoxia Yu, Jinsong Wang, Xiao Lin, Lihua Ge, Juan Du, Liping Wang, Shu Diao, Xiaomeng Lian, Songlin Wang, Rui Dong and Zhaochen Shan

Agonist-induced activation of human FFA1 receptor signals to extracellular signal-regulated kinase 1 and 2 through Gq- and Gi-coupled signaling cascades

FFA1 is abundantly expressed in the liver, skeletal muscle, monocytes and nervous system, but is particularly abundant in pancreatic β cells. It is widely believed that FFA1 exerts its regulatory roles in a va...

Authors: Jing Qian, Yuyang Gu, Chun Wu, Feng Yu, Yuqi Chen, Jingmei Zhu, Xingyi Yao, Chen Bei and Qingqing Zhu

miR-378 suppresses the proliferation, migration and invasion of colon cancer cells by inhibiting SDAD1

MicroRNAs (miRNAs) play important roles in the growth and metastasis of colon cancer. It is known that one set of miRNAs are dysregulated in colon cancer cells, but the mechanism of their role in cancer develo...

Authors: Mingxi Zeng, Linlin Zhu, Liangping Li and Changming Kang

Cells of human breast milk

Human milk is a complex fluid that has developed to satisfy the nutritional requirements of infants. In addition to proteins, lipids, carbohydrates and other biologically active components, breast milk contain...

Authors: Malgorzata Witkowska-Zimny and Ewa Kaminska-El-Hassan

LncRNA H19 promotes the differentiation of bovine skeletal muscle satellite cells by suppressing Sirt1/FoxO1

H19 is a well-characterized Long noncoding RNA (lncRNA) that has been proven to promote myoblast differentiation in humans and mice. However, its mechanism of action is still not fully interpreted.

Authors: Xiaochun Xu, Shengyue Ji, Weili Li, Bao Yi, Hengxin Li, Hongfu Zhang and Wenping Ma

Survival rates of homozygotic Tp53 knockout rats as a tool for preclinical assessment of cancer prevention and treatment

The gene that encodes tumor protein p53, Tp53, is mutated or silenced in most human cancers and is recognized as one of the most important cancer drivers. Homozygotic Tp53 knockout mice, which develop lethal canc...

Authors: Damian Strzemecki, Magdalena Guzowska and Paweł Grieb

Survivin, a molecular target for therapeutic interventions in squamous cell carcinoma

Squamous cell carcinoma (SCC) is the most common cancer worldwide. The treatment of locally advanced disease generally requires various combinations of radiotherapy, surgery, and systemic therapy. Despite aggr...

Authors: Zakir Khan, Abdul Arif Khan, Hariom Yadav, Godavarthi B. K. S. Prasad and Prakash Singh Bisen

ABCC6 knockdown in HepG2 cells induces a senescent-like cell phenotype

Pseudoxanthoma elasticum (PXE) is characterized by progressive ectopic mineralization of elastic fibers in dermal, ocular and vascular tissues. No effective treatment exists. It is caused by inactivating mutat...

Authors: Rocchina Miglionico, Angela Ostuni, Maria Francesca Armentano, Luigi Milella, Elvira Crescenzi, Monica Carmosino and Faustino Bisaccia

PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytes

Although the 3T3-L1 preadipocyte cell line represents an informative model for in vitro adipogenesis research, primary cultured cells are often needed to understand particular human or animal metabolic phenotypes...

Authors: Yong Pu and Almudena Veiga-Lopez

A simple and effective protocol for fast isolation of human Tenon’s fibroblasts from a single trabeculectomy biopsy – a comparison of cell behaviour in different culture media

Human Tenon’s fibroblasts (HTFs) play a crucial role in wound healing. They cause postoperative scarring of the filtering bleb and are thus responsible for trabeculectomy failure. This study aimed to find an e...

Authors: Agata Przekora, Tomasz Zarnowski and Grazyna Ginalska

The regulatory mechanisms of NG2/CSPG4 expression

Neuron-glial antigen 2 (NG2), also known as chondroitin sulphate proteoglycan 4 (CSPG4), is a surface type I transmembrane core proteoglycan that is crucially involved in cell survival, migration and angiogene...

Authors: Emmanuel Ampofo, Beate M. Schmitt, Michael D. Menger and Matthias W. Laschke

MicroRNA-210 induces endothelial cell apoptosis by directly targeting PDK1 in the setting of atherosclerosis

Atherosclerosis is a chronically inflammatory disease and one of the leading causes of deaths worldwide. Endothelial cell apoptosis plays a crucial role in its development. Several microRNAs (miRNAs) are repor...

Authors: Ying Li, Chunyan Yang, Lili Zhang and Ping Yang

MITF and PU.1 inhibit adipogenesis of ovine primary preadipocytes by restraining C/EBPβ

PU box-binding protein (PU.1) is a master gene of hematopoietic lineage and an important specific transcription factor in osteoclast lineage. There is proof of its expression in adipose tissue, and it is known...

Authors: ChongMei Ruan, Xiu Li, JunJie Hu, Yong Zhang and XingXu Zhao

Avoiding the side effects of electric current pulse application to electroporated cells in disposable small volume cuvettes assures good cell survival

The harmful side effects of electroporation to cells due to local changes in pH, the appearance of toxic electrode products, temperature increase, and the heterogeneity of the electric field acting on cells in...

Authors: Maciej Grys, Zbigniew Madeja and Włodzimierz Korohoda

p62 links the autophagy pathway and the ubiqutin–proteasome system upon ubiquitinated protein degradation

The ubiquitin–proteasome system (UPS) and autophagy are two distinct and interacting proteolytic systems. They play critical roles in cell survival under normal conditions and during stress. An increasing body...

Authors: Wei Jing Liu, Lin Ye, Wei Fang Huang, Lin Jie Guo, Zi Gan Xu, Hong Luan Wu, Chen Yang and Hua Feng Liu

Framework to function: mechanosensitive regulators of gene transcription

Mechanobiology has shifted our understanding of fundamental cellular and physiological functions. Changes to the stiffness of the extracellular matrix, cell rigidity, or shape of the cell environment were cons...

Authors: Megan Finch-Edmondson and Marius Sudol

IgG silencing induces apoptosis and suppresses proliferation, migration and invasion in LNCaP prostate cancer cells

Immunoglobulin G (IgG) has been implicated in the progression of various cancers. This study explored the role of IgG in the proliferation, apoptosis, cell cycle and in vitro invasive properties of LNCaP prost...

Authors: Yawen Xu, Binshen Chen, Shaobo Zheng, Yong Wen, Abai Xu, Kai Xu, Bingkun Li and Chunxiao Liu

The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro

The primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex inform...

Authors: Edyta Wrobel, Joanna Leszczynska and Edyta Brzoska

The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner

Cytoglobin (CYGB), a member of the globin family, is thought to protect cells from reactive oxygen and nitrogen species and deal with hypoxic conditions and oxidative stress. However, its molecular mechanisms ...

Authors: Xin Li, Shanshan Zou, Zhen Li, Gaotai Cai, Bohong Chen, Ping Wang and Wenqi Dong

Cordyceps bassiana inhibits smooth muscle cell proliferation via the ERK1/2 MAPK signaling pathway

Cordyceps belongs to a genus of acormycete fungi and is known to exhibit various pharmacological effects. The aim of this study was to investigate the effect of Cordyceps species on the p...

Authors: Enze Jin, Seongho Han, Mina Son and Sung-Whan Kim

Codon bias and the folding dynamics of the cystic fibrosis transmembrane conductance regulator

Synonymous or silent mutations are often overlooked in genetic analyses for disease-causing mutations unless they are directly associated with potential splicing defects. More recent studies, however, indicate...

Authors: Rafal Bartoszewski, Jaroslaw Króliczewski, Arkadiusz Piotrowski, Anna Janaszak Jasiecka, Sylwia Bartoszewska, Briana Vecchio-Pagan, Lianwu Fu, Aleksandra Sobolewska, Sadis Matalon, Garry R. Cutting, Steven M. Rowe and James F. Collawn

Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneo

How nutrition and growth factor restriction due to serum depletion affect trophoblast function remains poorly understood. We performed a proteomic differential study of the effects of serum depletion on a firs...

Authors: Susana Novoa-Herran, Adriana Umaña-Perez, Francesc Canals and Myriam Sanchez-Gomez

A computationally constructed ceRNA interaction network based on a comparison of the SHEE and SHEEC cell lines

Long non-coding RNAs (lncRNAs) play critical and complicated roles in the regulation of various biological processes, including chromatin modification, transcription and post-transcriptional processing. Intere...

Authors: Jiachun Sun, Junqiang Yan, Xiaozhi Yuan, Ruina Yang, Tanyou Dan, Xinshuai Wang, Guoqiang Kong and Shegan Gao

The role of Exo70 in vascular smooth muscle cell migration

As a key subunit of the exocyst complex, Exo70 has highly conserved sequence and is widely found in yeast, mammals, and plants. In yeast, Exo70 mediates the process of exocytosis and promotes anchoring and int...

Authors: Wenqing Ma, Yu Wang, Xiaomeng Yao, Zijian Xu, Liguo An and Miao Yin

Response gene to complement 32 regulates the G2/M phase checkpoint during renal tubular epithelial cell repair

The aim of this study was to evaluate the influence of RGC-32 (response gene to complement 32) on cell cycle progression in renal tubular epithelial cell injury.

Authors: Yun-lin Shen, Hua-jie Liu, Lei Sun, Xiao-ling Niu, Xin-yu Kuang, Ping Wang, Sheng Hao and Wen-yan Huang

BAG2 structure, function and involvement in disease

Bcl2-associated athanogene 2 (BAG2) shares a similar molecular structure and function with other BAG family members. Functioning as a co-chaperone, it interacts with the ATPase domain of the heat shock protein...

Authors: Lixia Qin, Jifeng Guo, Qian Zheng and Hainan Zhang

Obituary: remembering professor Arkadiusz Kozubek (1947–2016)

Authors: Aleksander F. Sikorski and Jerzy Gubernator

Posttranscriptional and transcriptional regulation of endothelial nitric-oxide synthase during hypoxia: the role of microRNAs

Understanding the cellular pathways that regulate endothelial nitric oxide (eNOS, NOS3) expression and consequently nitric oxide (NO) bioavailability during hypoxia is a necessary aspect in the development of nov...

Authors: Leszek Kalinowski, Anna Janaszak-Jasiecka, Anna Siekierzycka, Sylwia Bartoszewska, Marcin Woźniak, Dawid Lejnowski, James F. Collawn and Rafal Bartoszewski

Nuclear and nucleolar activity of linker histone variant H1.0

Histone H1.0 belongs to the class of linker histones (H1), although it is substantially distinct from other histone H1 family members. The differences can be observed in the chromosomal location and organizati...

Authors: Andrzej Kowalski

Changes in expression of cartilaginous genes during chondrogenesis of Wharton’s jelly mesenchymal stem cells on three-dimensional biodegradable poly(L-lactide-co-glycolide) scaffolds

In cartilage tissue regeneration, it is important to develop biodegradable scaffolds that provide a structural and logistic template for three-dimensional cultures of chondrocytes. In this study, we evaluated ...

Authors: Piotr Paduszyński, Ewelina Aleksander-Konert, Alicja Zajdel, Adam Wilczok, Katarzyna Jelonek, Andrzej Witek and Zofia Dzierżewicz

Effects of NH₄CL application and removal on astrocytes and endothelial cells

Hepatic encephalopathy (HE) is a complex disorder associated with increased ammonia levels in the brain. Although astrocytes are believed to be the principal cells affected in hyperammonemia (HA), endothelial ...

Authors: Miha Bartolić, Andrej Vovk and Dušan Šuput

Osteoporosis: the current status of mesenchymal stem cell-based therapy

Osteoporosis, or bone loss, is a progressive, systemic skeletal disease that affects millions of people worldwide. Osteoporosis is generally age related, and it is underdiagnosed because it remains asymptomati...

Authors: Jitrada Phetfong, Tanwarat Sanvoranart, Kuneerat Nartprayut, Natakarn Nimsanor, Kanokwan Seenprachawong, Virapong Prachayasittikul and Aungkura Supokawej

In vitro chondrogenesis of Wharton’s jelly mesenchymal stem cells in hyaluronic acid-based hydrogels

In this study, we evaluated the usefulness of two commercially available hyaluronic acid-based hydrogels, HyStem and HyStem-C, for the cultivation of Wharton’s jelly mesenchymal stem cells (WJ-MSCs) and their ...

Authors: Ewelina Aleksander-Konert, Piotr Paduszyński, Alicja Zajdel, Zofia Dzierżewicz and Adam Wilczok

A fermented bean flour extract downregulates LOX-1, CHOP and ICAM-1 in HMEC-1 stimulated by ox-LDL

This study focused on an extract from fermented flour from the Lady Joy variety of the common bean Phaseolus vulgaris. The extract, Lady Joy lysate (Lys LJ), is enriched in antioxidant compounds during the fermen...

Authors: Morena Gabriele, Laura Pucci, Margherita La Marca, Daniela Lucchesi, Clara Maria Della Croce, Vincenzo Longo and Valter Lubrano

WNT5A–ROR2 is induced by inflammatory mediators and is involved in the migration of human ovarian cancer cell line SKOV-3

Wnt5A, which is a member of the non-transforming Wnt protein family, is implicated in inflammatory processes. It is also highly expressed by ovarian cancer cells. ROR2, which is a member of the Ror-family of r...

Authors: Somayeh Arabzadeh, Ghamartaj Hossein, Zahra Salehi-Dulabi and Amir Hassan Zarnani

Nuclear expression of TCF4/TCF7L2 is correlated with poor prognosis in patients with esophageal squamous cell carcinoma

The prognosis for patients with esophageal cancer remains poor. Therefore, the identification of novel target molecules for the treatment of esophageal cancer is necessary. Here, we investigated the clinicopat...

Authors: Hideyuki Ishiguro, Takehiro Wakasugi, Yukio Terashita, Nobuhiro Sakamoto, Tatsuya Tanaka, Hiroyuki Sagawa, Tomotaka Okubo and Hiromitsu Takeyama

Identification of a novel deletion in the MMAA gene in two Iranian siblings with vitamin B12-responsive methylmalonic acidemia

Adenosylcobalamin (vitamin B12) is a coenzyme required for the activity of methylmalonyl-CoA mutase. Defects in this enzyme are a cause of methylmalonic acidemia (MMA). Methylmalonic acidemia, cblA type, is an...

Authors: Fatemeh Keyfi, Mohammad Reza Abbaszadegan, Arndt Rolfs, Slobodanka Orolicki, Morteza Moghaddassian and Abdolreza Varasteh

The crucial role of miR-126 on suppressing progression of esophageal cancer by targeting VEGF-A

miR-126 is a key regulator of oncogenic processes. It is functionally linked to cellular proliferation, survival and migration. Vascular endothelial growth factor A (VEGF-A), which is regarded as a tumorgenesi...

Authors: Ranran Kong, Yuefeng Ma, Jie Feng, Shaomin Li, Wei Zhang, Jiantao Jiang, Jin Zhang, Zhe Qiao, Xiaoping Yang and Bin Zhou

Toll-like receptor 2 and type 2 diabetes

Innate immunity plays a crucial role in the pathogenesis of type 2 diabetes and related complications. Since the toll-like receptors (TLRs) are central to innate immunity, it appears that they are important pa...

Authors: Zahra Sepehri, Zohre Kiani, Ali Akbar Nasiri and Farhad Kohan

Cellular and Molecular Biology Letters goes open access with BioMed Central

Authors: Aleksander F. Sikorski